1株H5N1亚型高致病力禽流感病毒人工感染鸭的细胞凋亡观察

Induction of Apoptosis by a Highly Pathogenic Avian Influenza Virus,A/duck/Guangdong/220/2004 (H5N1)in Experimentally Infected Ducks

TUNEL染色法和透射电镜观察表明,鸭静脉接种或眼-鼻-口腔-泄殖腔混合接种高致病力禽流感病毒A/duck/Guangdong/220/2004(H5N1)后,心、肝、脾、肺、肾、胰、肠、脑、胸腺和法氏囊均可发生细胞凋亡。其中,凋亡主要发生在胰腺泡上皮细胞、肾小管上皮细胞、肝细胞和肠黏膜上皮细胞;也有少量神经元、神经胶质细胞、心肌细胞、呼吸毛细管上皮细胞,胸腺、脾脏和法氏囊淋巴细胞发生凋亡。

Using terminal deoxyuridine transferase nick-end labeling（TUNEL）and electron microscopy technique, the apoptosis were investigated in ducks inoculated with a highly pathogenic avian influenza virus, A/duck/Guangdong/220/2004 （HSN1） by intravenous and ocular-nasal-oral-cloacal routes. The results demonstrated that the virus-induced apoptosis occurred in the pancreas, kidney, liver, heart, lung, intestine, cerebrum, cerebellum, thymus, bursa of Fabricius and spleen, etc. The predominent apoptotic cell types were pancreatic acinar epithelial cells, renal tubular epithelial cells, hepatocytes and intestinal epithelial cells. The minority of neurons, glial cells, cardiac muscle cells, epithelial cells of lung air capillary, lymphocytes in the thymus, bursa of Fabricius and spleen also experienced apoptotic changes.