摘要
右心肥大衰竭是导致腹水综合征(ascites syndrome,AS)患鸡发病的重要因素。作者通过免疫组化和原位杂交技术初步探讨c-fos和c-jun在AS肉鸡右心肥大和衰竭过程中的变化及维拉帕米对其表达的影响。结果发现:在22、29、36、43和50日龄时,与对照组肉鸡相比,低温组肉鸡右心室心肌细胞c-fos/c-jun蛋白和mRNA的平均光密度值显著或极显著(P<0.05;P<0.01)升高,其mRNA的阳性面积百分比也极显著升高(P<0.01);与低温组肉鸡相比,维拉帕米组肉鸡右心室心肌细胞c-fos/c-jun蛋白和mRNA的平均光密度值显著或极显著(P<0.05;P<0.01)降低,其mRNA的阳性面积百分比也极显著降低(P<0.01)。结果表明c-fos和c-jun癌基因在右心肥大和衰竭中起着重要作用。维拉帕米可有效抑制c-fos和c-jun癌基因的表达,有利于防止右心肥大和功能性衰竭。
Right ventricular hypertrophy and failure is an important factor in the development of ascites syndrome. In this study, the technology of immunohistochemistry and in situ hybridization were adopted to observe the changes of c-fos and c-jun in cardiac myocytes of broilers under low temperature and treated with verapamil. At the same time, the mechanism of c-fos and c-jun in right ventricular hypertrophy and failure was discussed. Compared with the controls, the broilers under low temperature showed that the average optical density of c-fos/c-jun protein and mRNA of right ventricular myocytes significantly increased on 22, 29, 36, 43 and 50 days of age, and the percentage of positive area about c-fos/c-jun mRNA expression also significantly increased. Compared with broilers under low temperature, the broilers given verapamil showed that the average optical density of c-fos/e-jun protein and mRNA of right ventricular myocytes significantly decreased on 22, 29, 36, 43 and 50 days of age, and the percentage of positive area about c-fos/c-jun mRNA expression also significantly decreased. These date demonstrated that c-fos and c-jun are important factors in right ventricular hypertrophy and failure. The verapamil can inhibit the expression of c-fos and c-jun, and prevent right ventricular hypertrophy and failure.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2009年第7期1082-1087,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金项目(30371063)
