白细胞介素10基因多态性及幽门螺杆菌感染与胃癌的相关性研究

Polymorphism of IL-10 and Helicobacter pylori infection on gastric cancer genesis

目的探讨白细胞介素10（IL-10）启动子区1082位点的单核苷酸多态性（single nucleotide polymorphisms，SNP）、不同类型幽门螺杆菌（Hp）感染与甘肃地区胃癌易感性之间的关系。方法采用聚合酶链反应-限制性片段长度多态（PCR．RFLP）和PCR产物直接测序技术分别检测来自甘肃地区人群137例胃癌患者及与其配比的144例对照个体及131例胃癌癌前病变IL-10-1982A／G基因多态性；采用免疫印迹技术对Hp进行毒株分型。结果①对照人群IL-10．1082位，鼠的AA、AG、GG3种基因型分布频率分别为76．4％、22．2％和1．4％；Hp感染情况为HpI型16．0％、Hp11型31．9％、阴性52．1％。②癌前病变组IL-10-1082位点的AA、AG、GG3种基因型分布频率分别为63．4％、32．8％和3．8％。AG＋GG基因型携带频率高于正常对照组（P=0．018），携带IL-10-1082AG＋GG基因型个体癌前病变的发病风险增高到1．87倍。③胃癌组IL-10-1082位点的AA、AG、GG3种基因型分布频率分别为58．4％、35．8％和5．8％；胃癌组IL-10—1082AG＋GG基因型携带频率显著高于正常对照组（P=0．010），携带IL-10—1082AG＋GG基因型个体胃癌的发病风险增高到2．31倍。④以IL-10—1082AA基因型并Hp免疫印迹阴性组为对照，AG＋GG基因型并Hp免疫印迹阴性个体、AG＋GG基因型并Hp感染个体、AG＋GG基因型并HpI型感染个体胃癌患病风险增高，其中AG＋GG基因型并HpI型感染个体的患病风险显著增高，为对照组的9．73倍。结论IL-10-1082位点A／G多态性与胃癌的遗传易感性相关。

ObjectiveTo analyze the relationship between 1082 site A/G polymorphism in interleukin-10 gene and different virulence factors of Helicobacter pylori in Gansu Province and susceptibility to gastric cancer genesis. Methods Polymerase chain restriction fragment length polymorphism and direct sequencing were performed to analyze the genotype of the A/G polymorphism in its-1082 site of promoter region, and immunoblotting was performed to test different virulence factors antibody of H. pylori. Results （1）The distribution frequency of AA, AG, GG genotypes were 76. 4% , 22. 2% , 1.4%, respectively in normal control. （2）The distribution frequency of AA, AG, GG genotypes were 63.4% ,32. 8% ,3.8%, respectively in the gastric precancerous lesion group. The frequency of AG ＋ GG genotype was statistically higher in the gastric precancerous lesion group compared to the control group （P = 0. 018 ） , the risk gastric precancerous lesion was 1.87 fold for individuals with the IL-10AG ＋ GG genotype. （3）The distribution frequency of AA, AG, GG genotypes were 58.4% ,35.8% ,5.8% , respectively in the gastric cancer group. The frequency of AG ＋ GG genotype was higher in the gastric cancer group compared to the control group （P = 0. 010） , moreover, individuals with the IL-10-1082AG ＋ GG genotype rose to 2. 31 fold risk for gastric cancer. @Compared with IL-10 the AA genotype and the H. pylori-immunoblotting negative individuals, there was an increased risk of gastric precancerous lesion and developing gastric cancer for those carrying both AG ＋ GG genotype and seropositive H. pylori I , with 9.73 fold risk. Conclusion There was a relationship between IL-10-1082 A/G polymorphism and susceptibility to gastric cancer.