晚期糖基化终末产物荧光光谱测量系统的设计

Design of Advanced Glycation Endproducts Fluorescence Spectrum Detecting System

提出了一种利用荧光光谱技术测量晚期糖基化终末产物的方法,阐述了该荧光光谱测量系统的测量原理,设计并搭建了该荧光光谱测量系统。利用该系统对365,370,375,380,385nm波段进行了激发光谱扫描测试,得出375nm为最佳激发波长;采用375nm的单色光作为激发光源,分别对正常人和糖尿病患者的皮肤进行了荧光光谱检测,发现两者在450nm附近的荧光存在明显的差异。实验结果证明该荧光光谱测量系统快速、无创、简单,可应用于对糖尿病、人体衰老、氧化应激等病情进行早期预测和诊断。

The method of making use of the technique of fluorescence spectrum to detect advanced glycation endproducts is dis- cussed in the present pape. The emphasis is on the principle and structure of the fluorescence spectrum detecting system. In the end, the authors made use of the system to detect the excitation spectrum at the wavelengths of 365nm, 370ran, 375um, 380nm and 385um, respectively, and the authors found that 375nm is the best excitation wavelength. At the same time, the emission spectrum was also detected on the skin of nondiabetic people and patients with diabetes respectively. The result of the experiment indicates that there is a difference distinctly at about 450um between them, and has proved the feasibility of the system. The detecting system does not need collecting blood sample, is a noninvasive detection technology, and avoids pain and infection to the patients. The process of detection is very rapid and convenient, and the repetitiveness is well. The patient can benefit from it to forecast and diagnose the state of illness such as diabetes, decrepitude and oxidative stress etc conveniently.