食管鳞状细胞癌中SDF-1的表达及DC抗肿瘤活性的影响

SDF-1 EXPRESSION AND AFFECTION TO THE ACTIVITY OF DC ANTI-TUMOR IN ESOPHAGEAL SQUAMOUS CELL CARCINOMA

目的探讨食管鳞癌中基质细胞衍生因子-1的表达与树突状细胞密度的相互关系及它们与患者临床病理特征的关系。方法运用免疫组化和原位杂交方法检测49例食管鳞癌组织中基质细胞衍生因子-1蛋白和mRNA、S-100蛋白标记的树突状细胞的表达,根据染色面积和染色强弱判断它们表达的水平。结果(1)肿瘤细胞分化和浸润深度与基质细胞衍生因子-1的表达呈正相关(rs分别为0.498、0.313,P<0.05);而与S-100蛋白阳性树突状细胞的密度呈负相关(rs分别为-0.501、-0.466,P<0.05)。(2)基质细胞衍生因子-1与树突状细胞密度间存在负相关性(rs=-0.619,P<0.05)。结论(1)基质细胞衍生因子-1蛋白的表达与食管鳞癌的临床病理特征存在明显相关性。(2)食管鳞癌中基质细胞衍生因子-1的表达可影响鳞癌组织中树突状细胞的密度,可能影响宿主的抗肿瘤能力及肿瘤的浸润转移。

Objective To investigate the relationship between expression of SDF - 1 and density of Dendritic cell （DC） in esophageal squamous cell carcinoma and their association with patients＇ clinicopathological features. Methods The expression of SDF - 1 and DC in 49 specimens of esophageal squamous cell carcinoma was detected by immunohistochemical technique and in situ hybridization, using anti - SDF - 1 monoclonal antibody, anti - S - 100 monoclonal antibody and labeled SDF - 1 mRNA probes. The results were evaluated according to the staining area and intensity . Results （ 1 ） SDF - 1 expression was actively associated with tumor grade , depth （ rs, respectively 0. 498,0. 313, P 〈 0.05 ） ; There was a close negative relation between the density of S - 100 positive DC and tumor grade , depth （rs, respectively - 0. 501, - 0. 466, P 〈 0. 05 ）. （2） There was a reverse correlation between the expression of SDF- 1 and the density of DC （rs = -0. 619 ,P 〈0.05 ）. Conclusion （1）SDF- 1 protein is associated with clinicopathological features of esophageal squamous cell carcinoma . （2） Expression of SDF - 1 might affect the density of DC and can affect the immune system and the invasion and metastasis of esophageal squamouscell carcinoma.