pAKT和VEGF在Ⅰ型子宫内膜癌的表达及其临床意义

Significance of pAKT and VEGF in TypeⅠEndometrial Carcinoma

目的探讨Ⅰ型子宫内膜癌组织磷酸化丝氨酸/苏氨酸激酶(pAKT)、血管内皮生长因子(VEGF)蛋白的表达及其临床意义。方法应用免疫组织化学SP法检测正常的子宫内膜、伴有不典型的复杂性增生子宫内膜组织和Ⅰ型子宫内膜癌中pAKT和VEGF的表达及其相关的临床指标。结果正常的子宫内膜和伴有不典型的复杂性增生子宫内膜组织中,pAKT和VEGF阳性表达率分别为20.0%(2/10)、40.0%(4/10)和40.0%(4/10)、60.0%(6/10),Ⅰ型子宫内膜癌pAKT和VEGF的阳性表达率分别为73.8%(31/42)和85.7%(36/42)。pAKT的阳性表达与肿瘤浸润深度和临床分期显著相关(P<0.05)。pAKT蛋白表达与VEGF蛋白表达密切相关(P<0.05)。结论pAKT和VEGF与Ⅰ型子宫内膜癌发生发展密切相关,因此阻断PI3K/AKT信号传导通路可能为子宫内膜癌的治疗提供新的靶点。

Objective To study the expression and significance of phosphorylated Akt（pAkt） and vascular endothyelial growth factor （VEGF） in type I endometrial carcinoma tissues. Methods Immunohistochcmical method was used to detect the expression and their clinical data of pAKT and VEGF in l0 normal cndometria, 10 complex atypical hyperplasia, and 42 type I endometrial carcinomas. Results The positive expression rates of pAKT and VEGF of normal endometria, complex atypical hyperplasia, and type I endometfial carcinoma were 20.0% （ 2/10 ）, 40.0% （ 4/10）, 40.0% （ 4/10）, 60.0% （ 6/10 ）, and 73.8% （ 31/42 ） , 85.7% （ 36/42 ） respectively. The positive expression of pAKT was correlated to the invasive depth and clinical staging. The expression of pAKT and VEGF was positively correlation. Conclusion The results shown that pAKT and VEGF are might had closed relation with carcinogenesis and progression of I endometrial carcinoma,it might be as a potentially useful target for therapeutic intervention in I endometrial carcinoma patients.