Tiam1,CD44v6和Ecadherin表达与胃癌组织病理生物学行为的关系

Expression of Tiam1,CD44v6 and Ecadherin in Gastric Carcinoma and its Relatinoship with the Pathobiological Behavior

目的探讨Tiam1、CD44v6和Ecadherin在胃癌组织中的表达及其与病理生物学行为的关系。方法应用RT-PCR和免疫组化SP法对56例胃癌和15例正常胃黏膜组织进行Tiam1、CD44v6和Ecadherin蛋白检测。结果与正常胃黏膜组织相比,Tiam1、CD44v6在胃癌组织中高表达(P=0.000,P=0.018),Ecadherin在胃癌组织中低表达(P=0.001);Tiam1、CD44v6、Ecadherin在胃癌组织中的表达与组织分化程度密切相关(P=0.022,P=0.016,P=0.018);Spearman等级相关分析显示,Tiam1与CD44v6的表达呈正相关(γs=0.576,P<0.01),Tiam1与Ecadherin的表达呈负相关(γs=-0.428,P<0.01)。结论Tiam1、CD44v6和Ecadherin的表达与胃癌的发生、发展和分化程度密切相关。

Objective To investigate the expression of Tiaml, CD44v6 and Ecadhefin in gastric carcinoma and its relatinoship with the pathobiological behavior. Methods The expression of Tiaml, CD44v6 and Ecadherin in 56 cases of gastric carcinoma and 15 cases normal gastric tissue were examined by immunohistochemical staining and RT-PCR. Results In comparion with those in normal gastric mucosa, the expression of Tiaml and CD44v6 in gastric carcinoma was significantly higher （P = 0. 000,P =0.018） ,and the expression of Eeadherin was lower（P =0. 001 ）. There was a closely correlation between the expression of Tiaml ,CD44v6 and Ecadherin in gastric carcinoma and its relationship with differentiated degree（P =0.022 ,P =0. 016, P = 0.018 ）. Spearman correlation analysis showed that there was a positive correlation between Tiaml and CD44v6（ γs = 0. 576, P 〈 0.01 ）, and an inverse correlation between Tiaml and Eeadherin （γs= - 0.428,P 〈 0.01 ）. Conclusion The expression of Tiaml, CD44v6 and Eeadherin in gastric carcinoma was closely correlated with carcinogenesis, development and differentiated degree.