高效毛细管电泳分离测定颠茄制剂中莨菪类生物碱

Systematic Separation and Quantitative Analysis of Tropane Alkaloids in Belladonna Preparation by High Performance Capillary Electrophoresis

选择毛细管区带电泳分离模式，以麻黄素为内标建立了适合莨菪类生物碱阿托品、东莨菪碱、山莨菪碱、樟柳碱化化分离与颠茄酊剂及片剂定量分析的高效毛细管电泳方法。电泳分离条件：毛细管40c×50μm，运行缓冲液（pH8.0）50mmol／L磷酸盐溶液-四氢呋喃（9:1），电迁移进样5kV×5s，运行电压11kV（＋）→（－），柱上检测UV200um，0．02AUFS，毛细管柱温24℃。本法简便、快速、重现性好，可用于含莨菪类生物碱中药制剂的质量控制。

<Abstact> A high performance capillary electrophoresis (HPCE) method was established for the sys-tematic separation of tropane aldaloids, i. e., atropine, scopolamine, anisodamine and anisodine, and quantita-tive analysis of Tincture and Tablet Belladonna,based on the mode of capillary zone clectrophoresis. A buffersolution composed of 50 mmol/L sodium dihydrogenphosphate at pH8. 0 containing 10% tetrahydrofuran wasfound to be the most suitab1e e1ectrolyte for this separation. The electromigration was performed on the 40 cm× 50 μm uncoated capillary with a 11 kV running voltage. The analytes were monitered by on-line ultraviolet(UV) detection at 200 nm and ephedrine was selected as internal standard- The analytical results demonstrat-ed the method was simple,rapid and well reproducible and could be used as a reliable tool for the quality con-trol of Chinese medicines containing tropane alkaloids.