多酚氧化酶固定化载体研究进展

A Review on Immobilization Matrix of Polyphenol Oxidase

固定化酶技术是当今酶工程的研究核心,它很好的实现了酶半衰期的延长、稳定性的提高、重复利用及产物与酶的分离。茶叶多酚氧化酶是茶树中含有的一类重要的氧化酶类,在茶叶加工、深加工以及茶饮料领域有着非常重要的作用。目前酶固定化的载体有无机载体、有机载体、复合型载体以及纳米载体。本文总结了酶固定化载体的性能要求,并概述了目前多酚氧化酶固定化酶的各种载体材料。具有功能基团的纳米载体材料将是多酚氧化酶固定化载体未来发展的主要方向。

Immobilized enzyme technology is the central part of enzyme engineering in current time. It has been well known that prolonging the half-life of enzyme, enhancing the stability, the reuse of enzyme and the separation of product and enzyme could be realized by this technology. Tea polyphenol oxidase is an important member of oxidases, it played an important role in tea-processing, tea refining processing and manufacture of tea beverage. The immobilized matrixs include inorganic-matrix, organic-matrix, complex-matrix and nano-matrix. The capability demand of immobilized enzyme matrixs, and various carriers applied to polyphenol oxidase immobilization were summarized in this paper. The nano-carrier with functional groups was regarded as the main matrixs of immobilized polyphenol oxidase in the future by the authors.