出生前后小鼠胸腺S-100蛋白表达的免疫组织化学分析

IMMUNOHISTOCHEMICAL ANALYSIS OF THE EXPRESSION OF S100 PROTEININ THYMUSES OF PRE AND POSTNATAL MICE

目的：研究出生前、后小鼠胸腺Ｓ１００蛋白的表达。方法：用免疫组织化学方法对小鼠胸腺Ｓ１００蛋白的表达时间、表达强弱及分布进行原位观察。结果：孕第１７、１９天胎鼠胸腺未见任何Ｓ１００蛋白表达；新生鼠及生后１～５周小鼠胸腺仅呈现极弱的Ｓ１００蛋白表达；生后６周小鼠胸腺Ｓ１００蛋白表达明显增强，并达到最高峰；６周后Ｓ１００蛋白表达又明显减弱。

ABSTRACT Objective:To study the expression of S100 protein in thymuses of prenatal and postnatal mice. Methods:By immunohistochemical method the time, intensity and distribution of the expression of S100 protein was observed in situ. Results:There was no visible expression of S100 protein in thymuses of 17day and 19day embryos. Only very weak expression of S100 protein could be observed in thymuses of newborn mice and postnatal mice ranging in age from 1 to 5 weeks. In thymuses of postnatal mice at the end of the 6th week, the expression of S100 protein became evident and reachs to the top. After the 6th week, the expression of S100 protein weakened obviously. Conclusion: The intensity of the expression of S100 protein in thymuses of mice was in relation with the increase of mice's age.