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大鼠全脑缺血再灌注损伤时海马细胞SOD、MDA及Cdk-5的变化

The Altreations of SOD, MDA and Cdk-5 in the Hippocampus of Global Ischemia-reperfurion Rat Brains
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摘要 目的:探讨急性全脑缺血再灌注损伤时大鼠海马超氧化物歧化酶(superoxidismutase,SOD),丙二醛(Malondialdehyde,MDA),细胞周期依赖性蛋白激酶-5(cyclin-dependent kinase 5,Cdk-5)的关系变化。方法:用三管结扎法建立急性全脑缺血再灌注大鼠模型,用免疫沉淀法测定Cdk-5的酶活性,用比色法测定SOD的活性及MDA的含量。结果:与对照组比较,在缺血和/或再灌注大鼠海马神经元内SOD活性降低(P<0.01),MDA含量明显升高(P<0.01),且伴有细胞内Cdk-5活性升高(P<0.05,P<0.01)。结论:本研究从整体水平上证实急性全脑缺血-再灌注时大鼠海马神经元Cdk-5活性升高,与氧化应激反应密切相关。 Objective：To explore the corralation of superoxidismutase, malondialdehyde and cyctin-dependent kinase 5（Cdk-5） in the hippocampus of global ischemia-reperfurion rat brains,Methods：A model of brain ischemia-reperfusion was established by clipped three arteries and then released to reperfuse blood into brain in rats.The rats were divided randomly into control,ischemia and ischemia- reperfurion（I-R）groups.SOD activity and MDA contain were detected by colorimetry, and the activity of Cdk-5 was measured by immtmoprecitation. Results：Compared with control group,SOD activity（P〈0.01,P〈0.01）was decreased and MDA contain was increased （P〈0.01,P〈0.01）in iscncmia group and in ischemia-reperfurion group（P〈0.0i）,and an etevated activity of Cdk-5 was inducedin ischemia group（P〈0.05）and in ischemia-reperfurion group（P〈0.01）.Conclusions：The higher level of Cdk-5 activity was tightly correlative with oxidative stress in the hippocampus of global ischemia-reperfurion rat brains.
作者 毕建波 胡日光 李亮 曲忠森
机构地区 潍坊医学院附属青岛市胶州中心医院 上海市浦东新区人民医院神经科
出处 《中国医药导刊》 2009年第7期1168-1169,共2页 Chinese Journal of Medicinal Guide
基金 中国博士后科学基金资助项目(20060390635)
关键词 缺血再灌注损伤 细胞周期依赖性蛋白激酶-5 超氧化物歧化酶 丙二醛 Ischemia-rcperfusion Injury Cyclin-dependent Kinase-5 SOD MDA
分类号 R743 [医药卫生—神经病学与精神病学]
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参考文献11

  • 1Shulda PK,Khanna VK,Ali MM.Anti-ischemic effect of curcumin in rat brain[J].Neurochem Res,2008;33(6):1036-1043.
  • 2Timsit S,Menn B.Cerebrel ischemia,cell cycle elements and Cdk5[J].Biotechnol J,2007,2(8):958-966.
  • 3Pigino G,Paglini G,Ulloa L,et al.Analysis of the expression,distribution and function of cyclin dependent kinase 5(cdk-5)in developing cerebellar macroneurons[J] J Cell Sci,1997;110(Pt2):257-270.
  • 4曲忠森,毕建波,李亮.全脑缺血再灌注损伤大鼠海马细胞周期依赖性蛋白激酶-5的变化[J].中国神经免疫学和神经病学杂志,2009,16(1):51-54. 被引量：2
  • 5Uno M,Kitazato KT,Suzue A,et al.Inhibitien of brain damage by edaravone,afree radical scavenger,can be monitored by plasma biomarkers that detect oxidative and astrocyte damage in patients with acute cerebral infarction[J].Free Radic Biol Med,2005,39(8):1109-1116.
  • 6Shea TB,Zheng YL,Ortiz D,et al.Cyclin-dependent kinase 5 increases perikaryal neurofilamant phosphorylation and inhibits neurofilament axouni transport in response to oxidative stress[J].J Nenrosci Res,2004;76(6):795-800.
  • 7Lee MS,Kwon YT,Li M,et al.Neurotoxicity inchced cleavage of p35 to p25 by calpain[J].Nature,2000;405(6784):360-364.
  • 8Patrick GN,Zukerberg L,Nikolic M,et al.Conversion of p35 to p25 deregulates Cdk5 activity and promotes neurodageneration[J].Nature,1999;402(6762):615-620.
  • 9Weishanpt JH,Kussmaul L,Grotsch P,et al.Inhibition of CDK5 is protective in necrotic and apoptotic paradigms of neuronal cell death and prevents mitochondrial dysfunction[J].Mol Cell Neurosci,2003;24(2):489-502.
  • 10Mitsios N,Peunucci R,Krupinski J,et al.Expression of cyclin-dapendunt kinase 5 mRNA and protein in the human brain following acute ischemic stroke[J].Brain Pathol,2007;17(1):11-23.

二级参考文献8

  • 1Timsit S, Menn B. Cerebral ischemia, cell cycle elements and Cdk5[J]. Biotechnol J, 2007, 2 (8) : 958- 966.
  • 2Pigino G, Paglini G, Ulloa L, et al. Analysis of the expression,distribution and function of cyclin dependent kinase 5 (cdk-5) in developing cerebellar macroneurons[J]. J Cell Sci, 1997, 110 (Pt2): 257-270.
  • 3Lee MS, Kwon YT, Li M, etal. Neurotoxicity induced cleavage of p35 to p25 by calpain[J]. Nature, 2000, 405(6784) :360-364.
  • 4Patrick GN, Zukerberg L, Nikolic M, et al. Conversion of p35 to p25 deregulates Cdk5 activity and promotes neurodegeneration [J]. Nature, 1999, 402 (6762) :615-620.
  • 5Wen Y, Yang SH, Liu R,et al. Cdk5 is involved in NFT-like tauopathy induced by transient cerebral ischemia in female rats [J]. Biochim Biophys Acta, 2007, 1772(4) :473-483.
  • 6Weishaupt JH, Kussmaul L, Grotsch P, et al. Inhibition of CDK5 is protective in necrotic and apoptotic paradigms of neuronal cell death and prevents mitochondrial dysfunction[J]. Mol Cell Neurosci, 2003, 24(2):489-502.
  • 7Mitsios N, Pennucci R, Krupinski J, et al. Expression of cyclin-dependent kinase 5 mRNA and protein in the human brain following acute ischemic stroke[J]. BrainPathol,2007, 17(1):11-23.
  • 8Yanpallewar SU, Hota D, Rai S, et al. Nimodipine attenuates biochemical, behavioral and histopathological alterations induced by acute transient and longterm bilateral common carotid occlusion in rats [J]. Pharmacol Res, 2004, 49(2) :143-150.

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中国医药导刊
2009年 第7期

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