抗坏血酸对大鼠神经干细胞分化影响的体外实验探讨

To Explore the Effects of Ascorbic Acid(AA) on Differentiation into Neurons from NSCs of Rats in Vitro

目的:探讨药物抗坏血酸对神经干细胞(NSCs)体外分化的作用。方法:体外培养和鉴定NSCs,细胞免疫学方法检测分化细胞中酪氨酸羟化酶(TH)表达。结果:对照组、AA20μm、AA50μm、AA100μm、AA150μm诱导7d后TH阳性细胞分别平均为1.5%、3.2%、5.5%、5.7%。结果显示AA处理后NSCs分化细胞明显TH阳性细胞表达率升高,与对照组比较有显著性差异(P<0.05)。结论:抗坏血酸AA体外对神经干细胞分化过程有重要的影响作用。

Objective：To explore the effects of AA on differentiation into neurons from NSCs in vitro. Methods： Culture and identify neural stem cells in vitro.We use immunocytochernistry to detect TH-positive neurons induced by the control and AA for 7days respectively.Results：The positive ratios of TH expression in induced cells by the control,AA20μm,AA50μm,AA100μm,AA150μm were 0.45%,1.5%,3.2%,5.5%,5.7% respectively.The positive ratios of TH expression in induced ceils by AA were more higher than by the control（P〈0.05）.Conclusion：The induction of AA on differentiation into neurons from NSCs in vitro is significant.