干化学法检测尿白细胞出现假阴性影响因素的探讨

The Desiccation of the False Negative Influencing Factor when Detecting the Urine WBC with the Dry-Chemical Method

目的:探讨用尿十一联试纸在尿液分析仪上检测白细胞时出现假阴性的影响因素。方法:对1500例于化学法检测白细胞阴性患者尿液再用Neubauer计数板计数白细胞。结果:尿液中含大剂量庆大霉素、试纸从尿中取出后立即测定、尿液中含大剂量先锋霉素Ⅳ、尿中偶见脓细胞、尿糖阳性时干化学法检测白细胞出现假阴性几率分别是93%、92%、90%、80%和54%。结论:尿液中含大剂量庆大霉素、试纸从尿中取出后立即测定、尿液中含大剂量先锋霉素Ⅳ、镜检尿中偶见脓细胞、尿糖阳性等因素都会使干化学法检测白细胞出现假阴性。

Objective：To discuss the influencing factors leading to the false negative results when detecting the urine WBC with the dry-chemical method.Methods：Count the WBC with the Neubauer counting board of 1500 urine samples of patients whose urine WBC were negative with the desiccation chemistry research method.Results：The false negative when detecting the urine WBC with the dry-chcmica method probability was respectively 93%,92%,90%,80% and 54% if the urine contained high-dose gentamicin,the urine were detected imediately out of the urine,the urine contained high-does Pioneer Ⅳ, pus cells were seen occasionally in the microscopic examination,glucose in urine was the masculine gender.Conclusions：The Urine contained high-dose gentamiein,reagent strips were detected imediately out of the urine,the urine contained high- dose Pioneer Ⅳ, pus cells were seen occasionally in the microscopic examination,glucose in urine was the masculine gender can cause the false negative when detecting the urine WBC with the dry-chemical method.