人胎盘绒毛层粘连蛋白及其受体的定位与定量研究

LOCALIZATION AND QUANTIFICATION OF LAMININ AND LAMININ RECEPTOR IN THE HUMAN PLACENTAL VILLI

目的：研究层粘连蛋白（ｌａｍｉｎｉｎ，ＬＮ）及其受体（ｌａｍｉｎｉｎｒｅｃｅｐｔｏｒ，ＬＮＲ）在早、中、晚期人胎盘绒毛的定位及其相对含量变化。方法：取早期、中期和晚期胎盘绒毛，常规制作石蜡组织切片，采用免疫组织化学ＡＢＣ法和图像分析技术进行ＬＮ及ＬＮＲ定位和定量测定。结果：ＬＮ阳性反应主要位于绒毛上皮基膜和绒毛血管内皮基膜；ＬＮＲ的阳性反应位于绒毛上皮合体滋养层表面及合体滋养层和细胞滋养层的细胞质内，细胞核呈阴性。ＬＮ及ＬＮＲ的含量以早期胎盘绒毛中最高，中期及晚期含量明显降低。结论：人胎盘绒毛含有ＬＮ及ＬＮＲ，随着胎盘周龄的增加。

ABSTRACT Objective: To study the localization and quantification of laminin (LN) and laminin receptor (LNR) in the human placental villi during early, middle and term pregnancy. Methods: LN and LNR in the human placental villi was measured using immunohistochemical ABC method and Q500 image analysis system. Results: LN positive immunostaining was found in placental villous epithelial basement membranes and vascular endothelial basement membranes. LNR positive immunoreactive substance was localized in the villous epithelial cellular plasma (including cytotrophoblast and syncytiotrophoblast ) and syncytiotrophoblastic cellular surfaces, but not in the nucleus. Along with placental development, the amount of LN and LNR decreased gradually. Conclusion: LN and LNR exist in the human placental villi, and their relative content decreased following the placental growth.