氟对鼠颅骨成骨细胞基质蛋白表达的影响

The effect of fluoride on bone extracellular matrix proteins expression in rat calvarial osteoblast in culture

目的研究低浓度氟环境对体外培养的胚胎鼠颅骨成骨细胞中的各类非胶原蛋白和胶原蛋白的表达。方法应用免疫细胞化学方法对成骨细胞中的骨涎蛋白，骨钙蛋白，骨桥蛋白，碱性磷酸酶和Ｉ型胶原表达进行检测。结果本研究可见胶原和非胶原蛋白在骨基质发育中的表达部位和作用有所不同；且在氟组的表达均要早于对照组。结论结果表明低浓度氟对较早期的成骨细胞具有一定的促增殖作用；同时氟可能还加速了成骨细胞的表型发育以及胶原和非胶原蛋白的合成。

Objective The expression of various non-collagenous proteins and collagen protein on the rat fetal calvarial osteoblast cultured in low doses fluoride condition in vitro had been studied. Methods Our study applied the immunohistochemistry technique to detect the expression of bone sialoprotein, osteocalcin, osteopontin, alkaline phosphatase and type I collagen expressed in the bone matrix. Results They demonstrated that there were difference in the location and function of the collagenous and non collagenous proteins in the development of bone matrix. Conclusion The present results revealed that fluoride might enhance the proliferation activity of osteoblast precursors; simultaneously, fluoride might accelerate the phenotypic development of osteoblast and the expression of collagenous and non-collagenous proteins. As a result,low doses fluoride played a favorable role in the development of bone extracellular matrix.