围排卵期卵泡内一氧化氮分析

Analysis of Nitric Oxide in Follicles Fluid During Periovulatory Period

目的：通过分析人围排卵期卵泡内一氧化氮（ＮＯ）水平与卵泡液量、白细胞介素１β（ＩＬ－１β）、雌二醇（Ｅ２）、孕酮（Ｐ）等卵泡内环境的关系及与血清Ｅ２、采卵数间的关系，探讨ＮＯ在卵泡发育、排卵中的作用。方法：卵泡液由２３名接受体外受精—胚胎移植（ＩＶＦ－ＥＴ）的志愿者提供，卵泡内ＮＯ水平应用荧光色谱分析法，ＩＬ－１β应用酶联免疫法，Ｅ２及Ｐ应用放免法测定。结果：卵泡内ＮＯ水平与ＩＬ－１β水平呈有意义正相关（ｒ＝０．４９Ｐ＜０．００１）；卵泡内ＮＯ水平与血清Ｅ２水平及采卵数呈有意义正相关（ｒ＝０．４６，Ｐ＜０．０５；ｒ＝０．６２，Ｐ＜０．０１）。结论：在人的围排卵期，卵泡内有一定水平的ＮＯ，并可能受ＩＬ－１β的调节；

Objective:This paper was to study the effect of nitric oxide(NO) on follicular development and ovulation.Methods:Follicular fluid was individually collected from 23 women undergoing IVF treatment. The concentration of NO,IL 1β,E 2 and P were assayed with Fluormetry, ELISA,and RIA.Results:Follicular NO was sighificantly[KG2〗correlated[KG2〗with[KG2〗follicular IL 1β Concentrations(r=0.49 P<0.001);and was also significantly and positively correlat with the number of oocytes retrieved(r=0.62 P<0.01) and serum E 2(r=0.46 P<0.05).Conclusions:A certain level of NO was present in follicular fluid during human Periovalatory period. It might be regulated by IL 1β. NO could erect an important function in follicular derelopment and ovulation.