老年大鼠肝脏超微结构加龄变化的研究

AGING CHANGES OF ULTRASTRUCTURE OF OLD RAT LIVER

对雄性18月、25月、28月及31月龄大鼠肝脏超微结构的加龄变化进行了透射电镜的观察.发现从25月龄开始肝细胞核有的出现异染色质增多、边集,粗面内质网逐渐减少.线粒体基质深浅不一,凡基质变深者其嵴亦由板状变为泡状,最深者嵴消失.在有脂肪变性的肝细胞,其线粒体大小悬殊,形态多样,基质增深,嵴多变为泡样;内质网消失.胆小管内与窦周隙中的微绒毛减少,而窦周隙中的胶原原纤维则增多.31月龄大鼠除上述现象外,窦周隙中不但微绒毛消失,而且内皮细胞结构不清,在其外与肝细胞膜的窦周隙面均出现不规则的基膜样结构.

Aging changes of male rat liver (25, 28 and 31 m) were observed by Electron microscopy. The results were as follows- From 25 m on, the riucli of some liver cells possessed more peripheral heterochromatin and less RER, and the form and the density of the mitochondria became diverse. Among those whose matrix became dark, their cristae were distinct or even vanished. In liver cells with fat degeneration, the mitochondria varied both in form and size, possessed vesicular cristae and dense matrix, but endoplasmic reticulium disappeared. The microvilli both in the bile canaliculi and the Disse's space decreased in number, but collagenous fibrils increased in number in the Disse's space. From 31m on the microvilli in the Disse's space vanished and the endothelium became indistinct. A structure like basement membrane appeared both out of the hepatocyte and the endothelium.