应用若丹明荧光素标记的人抗汉滩病毒抗体检测小兽中的汉滩病毒感染

Detection of Hantavirus Infection in Small Mammals by Using RBITCLabeled Human Antihantavirus Antibody.

１９９６年我们用异硫氰酸若丹明Ｂ（ＲＢＩＴＣ）标记的人抗汉滩病毒（ＨＴＮＶ）抗体，对广安县肾综合征出血热疫区小兽肺组织进行了直接免疫荧光试验，检出两只褐家鼠为ＨＴＮＶ阳性。与１９９５年用ＦＩＴＣ标记抗体进行的间接免疫荧光试验的比较显示，１９９６年和１９９５年的动物带毒率和带毒指数均无显著差异，结果表明。

A human antihantavirus antibody was prepared and labeled with RBITC. In 1996,an immunofluorscence antibody assay(IFA) with this antibody was performed to detect hantavirus infection in the lung tissues from small mammals in a hemorrhagic fever with renal syndrome(HFRS) endemic region of Guangan county, Sichuan province The lungs of two rats(Rattus norvegicus) among the mammals were found to be hantavirus antibody positive A comparison between the abovementioned results and those obtained in 1995 by indirect IFA using FITClabeled sheep antihantavirus antibody showed that there were no significant differences between both the hantavirus infection rates and indices among animals in 1996 and 1995. The data also indicated that the RBITClabeled human antihantavirus antibody may be used to detect hantavirus infection in small animals in HFRSendemic regions.