绿脓杆菌外膜微孔蛋白的分离与纯化

Isolation and Purification of Porin in Pseudomonas aeruginosa

选用绿脓杆菌 PAO1菌株 ,以 EDTA-溶菌酶法与超速离心法提取其外膜蛋白 ,用SDS-聚丙烯酰胺凝胶电泳测得其外膜主要蛋白的分子量 。

Pseudomonas aeruginosa PA01 was used in this study. Isolation of outer membrane protein was accomplished by treating the cell envelope with EDTA and lysozyme, followed by centrifugation. The molecular weight of the major outer membrane protein was analyzed by SDS PAGE. The purification of the porins in Pseudomonas aeruginosa was achieved by high performance ion exchange liquid chromatography.