冠心病患者阿司匹林抵抗与识别活化血小板GPⅡ bⅢ/a复合物单克隆抗体(PAC-1)的相关性研究

Study on Correlation Between Aspirin Resistance and PCA-1 of Platelet in Patients with Coronary Heart Disease

目的探讨冠心病患者阿司匹林抵抗现象(AR)与PAC-1的相关性。方法经冠脉造影确诊为冠心病患者80例,服用阿司匹林100 mg/d≥7d,以二磷酸腺苷(adenosine diphosphate,ADP)为诱导剂测定血小板聚集率,以ADP诱导的血小板聚集率≥70%定义为AR,<70%为阿司匹林敏感(aspirin sensitive,AS)。同时测定患者血小板PAC-1的表达阳性率。结果AR的发生率为20%,两组患者服药后血小板PAC-1表达有显著性差异,AR组患者服药后PAC-1水平显著高于AS组(P<0.05)。结论冠心病患者AR的发生率为20%,PAC-1可作为检测AR的有效指标。

Objective To investigate the correlation between aspirin resistance （AR） and PAC-1 expression of platelet in patients with coronary heart disease （CHD）. Methods 80 CHD patients diagnosed by coronary artery angiogram took aspirin 100 mg/d for ≥7 d, and then their blood samples were collected for determination of optical platelet aggregation index using adenosine diphosphate（ADP）. AR was defined as a state in which aggregation of ≥70% with ADP was found. We detected platelet aggregation （PAG） induced with adenosine diphosphate （ADP） and expression of PCA -1 in platelet. Results 20% of patients showed AR. The expression of PCA- 1 was significantly higher in AR patients than in AS （P 〈 0.05 ） . Conclusion The AR accounts for 20 % of all. PAC- 1 could be considered as the sensitive index of platelet activation and be used in the evaluation of anti-platelet therapy.