摘要
采用KyteDoolittle氨基酸亲水性标准及Bairoch的GGBSM方案对人C5aR分子的亲水性基序、B细胞表位及二级结构进行了预测,并用吴氏建立的B细胞表位预测方法进行评述。结果显示,不同的预测方案所得结果一致,人C5aR分子中,构成螺旋结构,伸展构象及无规卷曲的氨基酸残基数比例分别为33.7%,34.6%,31.7%。三个高亲水性区域为第15~18位的DDKD,第195~200位的DKRRER和第330~333位的RESK,其Ah值分别为3.0、3.0、2.32。其平均抗原性指数AI值分别为0.064、0.091、0.070。N-端第9~30氨基酸序列的AI=0.042,是B细胞优势表位区,以此制作短肽单克隆抗体是可行的。
The hydrophilicity profile,Bcell dominant epitopes and secondary structures of the receptor for human C5a were predicted by using kyte & Doolittle′s method and Bairoch′s method.The results were as followes:The Ah(Average hydrophilicity)of D15DKD,D195KRRER,R330ESK profile,Which were the high hydrophilicity profile,were 3.0,3.0,2.32,respectively.The percentage of amino acid in Helical,Extended and coil structure is 33.7%,34.6%,31.7%,respectively.The Nterminal No.9~30 was the predominant epitope of the Bcells.This poly peptide could be made for the production of sitedirected monoclonal antibody.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1998年第3期153-156,共4页
Immunological Journal
基金
国家自然科学基金