转染B7-1基因至鼻咽癌细胞株及其诱导的细胞免疫应答

INDUCTION OF CELLULAR IMMUNITY BY B71 GENE TRANSFECTED NASOPHARYNGEAL CARCINOMA CELL STRAIN

通过包装细胞把逆转录病毒载体ｐＬＸＳＮ／Ｂ７－１导入鼻咽癌细胞株ＣＮＥ１，利用ＲＴ－ＰＣＲ及ＦＡＣｓ等技术证实Ｂ７－１的表达情况，同时检测了ＣＮＥ１细胞表面ＭＨＣＩ抗原。在此基础之上，观察了表达Ｂ７－１的ＣＮＥ１细胞（ＣＮＥ１／Ｂ７－１）对健康个体ＰＢＬ（外周血淋巴细胞）的增殖及细胞因子分泌的影响，结果表明：ＣＮＥ１／Ｂ７－１可以促进ＰＢＬ的增殖及细胞因子的分泌，且增高水平的细胞因子主要是ＩＬ－２和ＩＦＮ－γ。提示：ＣＮＥ１／Β７－１诱导的免疫应答以细胞免疫为主。

The nasopharyngeal carcinoma cell strain CNE1 was transfected with retrovirus vector pLXSN containing the B71 cDNA sequence.The expression of B71 was evaluated by the technique of RTPCR and FACs,and the expression of MHC I was also analyzed.Furthermore we observed that the B71 expressing CNE1 not only could increase the proliferation of PBL from health donors,but also enhance secretion of some cytokines,the enhanced cytokines were mainly IL2 and IFNγ which typically secreted by the Th1 cells.This results indicate that transfected CNE1 cells mainly induce cellular immunity.