HLA-DRB1多态性对LCL TNF分泌能力的影响

INFLUENCE OF HLADRB1 GENETIC POLYMORPHISM ON TNF PRODUCTION IN B LYMPHOBLASTOID CELL LINES

通过对２７个已确定ＨＬＡ－ＤＲＢ１基因型别的ＬＣＬ进行ＴＮＦ生物活性的检测，结果发现：ＴＮＦ的表达水平与特定的ＨＬＡ－ＤＲＢ１等位基因型别关联，携带ＨＬＡ－ＤＲＢ１＊０７０Ｘ的个体，其ＬＣＬ经ＬＰＳ刺激后，ＴＮＦ生物活性水平比不携带ＨＬＡ－ＤＲＢ１＊０７０Ｘ的个体低（Ｐ＜０．０１），且以ＨＬＡ－ＤＲＢ１＊０７０Ｘ纯合子细胞表达量最低，杂合子次之。提示：ＨＬＡ－ＤＲＢ１遗传多态性参与调控ＬＣＬ的ＴＮＦ表达水平，影响ＴＮＦ的产生可能是ＨＬＡ复合体发挥免疫调节的重要因素之一。

The cytotoxicity of 27 HLAdefined normal LCL supernatants against TNFsensitive cell line L929 was tested.The results showed that culture supernatants of LPSstimulated LCLs of HLADRB1070X genotype individuals had a significantly lower TNF bioactivity than those from nonHLADRB1070X genotype individuals (P<0.01)esp.a lowest in DRB1070X homozygous cell lines.These findings suggest that TNF is one of the important factors participating in regulation of the immune response controlled by MHC,and variation of TNF production among individuals may be genetic trait associated with HLA polymorphism.