白芷香豆素的镇痛作用部位及其机制

Studies on analgesic site and mechanisms underlying of Coumarins of Angdicae Dahuricae

目的观察白芷香豆素(Coumarin of Angelicae Dahuricae,CAD)的镇痛作用部位及其机制。方法利用小鼠福尔马林实验及侧脑室注射CAD对小鼠热板痛反应潜伏期的影响以分析CAD的镇痛作用部位;观察CAD对甲醛所致伤害性疼痛模型小鼠血清一氧化氮(NO)和脑组织中β-内啡肽(β-EP)含量的影响。结果CAD(30,60,120 mg/kg)不同程度地抑制了小鼠福尔马林实验第一和第二时相反应。侧脑室注射CAD(6 mg/kg)明显延长小鼠热板痛反应潜伏期。CAD(30,60,120 mg/kg)连续给药4 d,使甲醛所致伤害性疼痛模型小鼠血清NO含量和脑中β-EP含量明显下降。结论CAD对物理、化学等伤害性刺激具有明显镇痛作用,作用部位可能既在中枢也在外周,镇痛机制可能与抑制NO的合成或释放有关。

Objective To observe analgesic site and mechanisms underlying of Coumarins of Angdicae Dahuricae （CAD）. Methods The anti-nocieeptive site of CAD was analysed with mice formalin test and mice hotplate test after intraeerebroventricular （icv） administration;the concentration of nitric oxide （NO） in serum and β-EP in brain of mice were measured with nitric reductase method and radioimmunoassay （RIA） respectively. Results CAD （30,60,120 mg/kg） reduced the firstand second-phase responses of mice formalin test compared with control group. After one time icy administration of CAD （6 mg/kg） for mice, hotplate latency were significantly prolonged. The concentration of NO in serum and β-EP in brain of mice induced by formalin were both decreased. Conclusions CAD could induce definite analgesic effect, which may be related to central and peripheral mechanisms.