实验动物组织血管铁质反应与胶原、弹性纤维和肌纤维的组合染色法

A Compound Staining of the Collagen,Elastic and Muscular Fibers and Iron in the Blood Vessel

在进行实验动物组织染色反应中,为了证明血管铁质物质与胶原、弹性纤维和肌纤维的分布情况,选用丽春红S苦味酸(Ponceau S picric acid)、间苯二酚碱性复红(Resorein basic fuchsin)与Perls铁法(简称P-PA-R-BF-P法),对大鼠组织进行组合染色,能够较好显示组织血管中铁质物质与胶原、弹性纤维和肌纤维,铁质物质呈蓝色,胶原纤维呈红色,弹性纤维呈棕色,肌纤维呈黄色。这是一种相互对比清晰的组合染色方法。

A compound staining method composed of the Ponceau S picric acid, Resorein basic fuchsin and Perls stainings was used to show the distribution of collagen, elastic and muscular fibers and iron in the blood vessel. It was found that the compound staining displayed the red collagen, brown elastic and yellow muscular fibers, as well as green iron. The compound staining method could clearly identify different fibers in the blood vessels.