IL-4对重型肝炎患者外周血单个核细胞IL-1α表达调节的研究

Study on regulation of IL 4 on expression of IL 1α in PBMCs from patients with subacute and chronic severe hepatitis

目的评价ＩＬ－４在重型肝炎治疗中的潜在应用价值。方法应用免疫组化法和半定量ＲＴ－ＰＣＲ系统地观察ＩＬ－４对重型肝炎患者ＰＢＭＣｓＩＬ－１α表达的影响。结果ＩＬ－４在蛋白质和ｍＲ－ＮＡ水平明显抑制ＬＰＳ诱导的亚急性和慢性重型肝炎患者ＰＢＭＣｓＩＬ－１α的产生，这种抑制作用呈剂量和时间依赖的方式。ＩＬ－４于１００Ｕ／ｍｌ时开始出现抑制效应，于１０００Ｕ／ｍｌ时抑制作用接近最大。虽然ＩＬ－４对慢性重型肝炎患者ＰＢＭＣｓＩＬ－１α的抑制作用略低，但与未经ＩＬ－４处理的ＰＢＭＣｓ相比较，ＩＬ－１α的表达水平仍明显下降。结论ＩＬ－４对亚急性和慢性重型肝炎ＰＢＭＣｓＩＬ－１α的表达均具有较强的下向调节作用，在重型肝炎的治疗上可能具有潜在的应用价值，值得进一步研究。

Objective To estimate the potential clinical value of IL 4 in the treatment of severe hepatitis. Methods In the present study, we observed the effect of IL 4 on expression of IL 1α in peripheral blood mononuclear cells (PBMCs) from patients with severe hepatitis at cellular and molecular levels using immunohistochemical assay and the technique of semiquantitative reverse transcriptional polymerase chain reaction (RT PCR).The isolated PBMCs were cultured in the presence of increasing concentration of IL 4 or IL 4 was added to cultures at different time courses after the challenge of LPS. Results It was found that IL 4 in dose and time dependent manner down regulated the LPS induced production of IL 1α in PBMCs from patients with severe hepatitis at the level of protein and mRNA. The inhibitory effect of IL 4 could be observed at the concentration of 100U/ml and nearly got to the climax at 1 000U/ml.Although the suppression of IL 4 on the production of IL 1α in PBMCs from chronic severe hepatitis was slightly weaker than on that from subacute severe hepatitis,there existed significant difference when compared with the control PBMCs untreated with IL 4. Conclusion