HBsAg阳性肝病患者HCV感染率及其在血中的存在形式

The Incidence of HCV Infection and It's Existing Formsin Patients with HBsAg Positive Liver Diseases

目的：探讨ＨＢｓＡｇ阳性肝病患者合并ＨＣＶ感染后对肝脏病变的影响以及血清中ＨＣＶ存在的形式。方法：被测对象为１６８例ＨＢｓＡｇ阳性肝病患者（肝炎后肝硬化１０４例，原发性肝细胞癌６４例），采用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）检测血清中ＨＣＶＲＮＡ，采用免疫沉定法判断ＨＣＶ在血清中存在的形式。结果：ＨＢｓＡｇ阳性肝病患者合并ＨＣＶ感染者为２０．８％，与无ＨＢＶ感染人群ＨＣＶ感染率２．２％比较有显著性差异。ＨＣＶ在血清中的存在形式７７％呈游离状态，２３％既有游离状态，又有免疫复合物的形式。ＨＢｓＡｇ阳性脏病患者合并ＨＣＶ感染者肝功能损害明显加重，肝癌并发率为２６．６％。

Objective:This paper was to study the influence of HCV infection on the liver function of patients with HBsAg positive liver diseases, and the existing form of HCV in sera. [WT5”HZ〗Methods:[WT5”BZ〗Total of 168 patients with HBsAg positive liver diseases were studied. HCV RNA and the existing HCV forms in sera were determined with reverse transcriptionpolymerse chain reaction(RTPCR) and immunoprecipitation method respectively.[WT5”HZ〗Results:The[KG2〗incidenceof HCV infection in HBsAg positivepatients was 20.8%, which was higher than that(2.2%) of people without HBV infection. In 77%(27/35) patients, the HCV was in free form and in 23%(8/35) patients,the HCV was in both free and immunocomplex forms in sera. The liver functions were severely damaged and the hepatocarcinoma incidence was high(26.6%) in patients with combinated HBV and HCV infection.Conclusions:The findings suggest that the severe damage of liver function and the high incidence of hepatocarcinoma were closely related to the synergistic action of HBV and HCV.