转化生长因子-β及胰岛素样生长因子-Ⅰ修复关节软骨缺损的实验观察

Effect of transforming growth factor-β and insulin-like growth factor-Ⅰ on the repair of articular cartilage defects

目的观察转化生长因子-β(transforming growth factor-β,TGF-β)、胰岛素样生长因子-Ⅰ(insulin-like growth factor-Ⅰ,IGF-Ⅰ)对关节软骨缺损修复的作用。方法采用组织工程方法制备骨基质明胶(BMG)软骨细胞移植物。将40只4月龄的新西兰兔随机分为TGF-β组、IGF-Ⅰ组、TGF-β联合IGF-Ⅰ组、空白对照组(前三组为实验组)。各组制备关节软骨缺损模型,实验组兔膝关节腔注射对应等量人重组蛋白,对照组注射等量盐水。术后行组织学观察及免疫组化检测。结果TGF-β联合IGF-Ⅰ组软骨细胞生长较快,术后24周修复的软骨组织HE染色与正常关节软骨一致,软骨细胞呈柱状排列,免疫组化见Ⅱ型胶原染色较深;TGF-β组、IGF-Ⅰ组术后24周部分软骨细胞呈柱状排列,免疫组化见Ⅱ型胶原染色较浅;空白对照组未修复。结论联合应用TGF-β及IGF-Ⅰ可较好促进关节软骨缺损修复,其作用优于两者单独应用。

Objective To observe the effect of transforming growth factor- β and insulinlike growth factor- Ⅰ on the repair of articular cartilage defects. Methods Bone matrix gelatin （BMG） engineered chondrocytes were implanted into the articular cartilage defects on the allogeneic rabbit knee joint. TGF- β and IGF- Ⅰ were injected into the knee cavity in the experiment group, saline was injected in the control group. The histologic staining and immunohistochemistry were performed postoperatively. Results Chondrocytes arrangement, histologic staining, and expression of Type 2 collagen of the repaired cartilage tissues in the TGF-β combining with IGF-Ⅰ group were found to be better than the other groups. The repaired cartilage tissues in TGF- β combining with IGF-Ⅰ group were found to be similar to the normal articular cartilage 24 weeks after operation. The blank control group was not repaired.Conclusions TGF-β combining with IGF- Ⅰ can promote the repair of articular cartilage defects, and the effect is better than TGF- β and IGF- Ⅰ alone respectively.