抗HBs-HBsAg复合物诱生体液免疫应答的研究

Humoral immune responses induced by anti HBs HBsAg immunogenic complexes (IC) in BALB/c mice

目的研究抗ＨＢｓ－ＨＢｓＡｇ复合物在ＢＡＬＢ／ｃ小鼠诱生体液免疫应答的特点。方法分别用鼠抗ＨＢｓ－ＨＢｓＡｇ免疫原性复合物（ＩＣ）或单独ＨＢｓＡｇ免疫ＢＡＬＢ／ｃ小鼠（加或不加氢氧化铝），分析其诱生的抗ＨＢｓＩｇＧ效价及ＩｇＧ亚类。结果用ＩＣ加氢氧化铝为佐剂免疫小鼠诱生的抗ＨＢｓ中以ＩｇＧ１亚类为主，而用ＨＢｓＡｇ免疫鼠则以ＩｇＧ２ｂ亚类较高。ＩＣ不加氢氧化铝佐剂诱生的抗ＨＢｓ中ＩｇＧ１及ＩｇＧ２ａ均高于单独ＨＢｓＡｇ免疫鼠。研究还显示，抗ＨＢｓＩｇＧＦｃ段是巨噬细胞（ＭΦ）等抗原提呈细胞摄取ＩＣ的主要途径。结论抗ＨＢｓ－ＨＢｓＡｇ复合物免疫诱生的体液免疫应答较单独ＨＢｓＡｇ免疫强。抗ＨＢｓ主要通过Ｆｃ段的介导作用，改变了机体抗原提呈细胞对ＨＢｓＡｇ的摄取、加工及抗原提呈，并可增强ＨＢｓＡｇ的免疫原性。

Objective To study the anti HBs IgG and IgG subclasses induced by anti HBs HBsAg immunogenic complexes in BALB/c mice. Methods [WT5”BZ] Mouse anti HBs HBsAg complexes or goat anti HBs HBsAg complexes were separately used to immunize BALB/c mice,and the mice were boostered with IC or HBsAg alone three weeks later .ELISA assay was used to detect the anti HBs IgG and IgG subclasses. [WT5”HZ〗Results Immunization employing mouse anti HBs HBsAg complexes adsorbed to alum resulted in predominantly IgG1 response,whereas without adjuvant,both IgG1 and IgG2a antibodies were higher than those induced by HBsAg alone.Data also indicated that the Fc fragment of anti HBs IgG was necessary for increased uptake of anti HBs HBsAg complexes by antigen presenting cells. [WT5”HZ〗Conclusions Mouse anti HBs HBsAg complexes induced stronger humoral immune responses in BALB/c mice than that did by HBsAg alone. The Fc fragment of anti HBs mediated changes in uptake,processing and presentation of HBsAg by APC,which may increase the immunogenicity of HBsAg.