慢性乙型肝炎和肝病中Fas和FasL表达的原位研究

In situ investigation of Fas/FasL expression in chronic hepatitis B and liver diseases

目的了解慢性乙型肝炎和肝病时介导凋亡的Ｆａｓ／ＦａｓＬ表现。方法以免疫组化法原位检查各种慢性肝病４５例的活检肝组织。结果肝内浸润的淋巴细胞中检出ＦａｓＬ，肝细胞Ｆａｓ／ＦａｓＬ表达与炎症活动性一致，多分布在界面性炎症区。肝细胞表达ＦａｓＬ，可能也发挥细胞毒效应。结论新的发现是ＦａｓＬ可在肝细胞结节和肝癌细胞表达，提示有细胞毒效应的ＦａｓＬ在ＨＢ相关慢性肝病中有发病学意义。肝癌细胞中未检出Ｆａｓ而检出ＦａｓＬ。

Objective To elucidate the appearance of Fas/FasL induced apoptosis in hepatitis B virus related chronic liver diseases.Methods Forty five biopsied liver samples from 44 corresponding cases were investigated by the technique of in situ immunohistochemistry. Results The FasL was detected in the infiltrating lymphocytes.The Fas and FasL expressions were closely correlated with the inflammatory activity.The Fas/FasL signals mostly distributed in the interface of inflammatory region.The hepatocyt expressed FasL which might induce cytotoxicity. Conclusion A new discovery was that FasL was expressed in liver nodules and hepatocellular carcinoma cells,suggesting the FasL might induce cytotoxicity and was involved in the pathogenesis of HBV related chronic liver diseases.In carcinoma cells,Fas was not detected whereas FasL was detected,implicating a mechanism that tumor cells evaded immune attacks.