猕猴桃根抗肝损伤活性的研究

Study on anti-hepatic injury activity of actinidia delicilsa root

采用CCl4引起小鼠急性肝损伤建模,并通过测定血清中ALT和AST两种转氨酶活性的变化确定最佳的药剂量为60 mg/kg。然后在最佳药剂量的条件下分别给予急性肝损伤小鼠猕猴桃根不同部分的萃取物进行活性筛选。结果标明:猕猴桃根的乙醇提取物经乙酸乙酯萃取后,再经大孔树脂(AB-8)柱乙醇梯度洗脱,其中45%、60%的乙醇洗脱物对CCl4引起的急性肝损伤小鼠血清中的ALT和AST的活性有显著的降低作用,抑制率分别为63.2%和51.4%。利用MCI和ODS色谱柱层析对有活性的乙醇洗脱物进行分离,最终得到5种乌苏酸型化合物。

The model was established by using carbon tetrachloride to caused acute hepatic injury of mice, and the optimal dose of 60mg/kg was determined by measuring the activity of two aminotransferases （ALT and AST） in serum. Under the condition of optimal dose, ex tracts of different parts were given to the mice. The alcohol extractive of actinidia root was leached by acetidin, then gradient elusion on the macroporous absorption resin by the alcohol, and the extractive was separated into four parts （ Ⅰ , Ⅱ , Ⅲ ,Ⅳ）. Then, research was carried on the activity of these. The result showed that part n and Ⅲ were with the activity of decreasing the ALT and AST. The antihepatic injury rates were 63.2% and 51.4%, respectively. The sec tion Ⅲ is the most active one as so to be selected for the next further separation and distillation. By the means of MCI and ODS column chromatography, 5 compounds had been separated finally.