摘要
以商品茶多酚为原料,先用聚酰胺柱层析预分离表没食子儿茶素没食子酸酯(EGCG),再用硅胶柱层析制备高纯度的EGCG。采用此工艺条件,利用较低等级的商品茶多酚(70%TP)可以制备出纯度达98%以上的EGCG。
A method is described for purification of epigallocatechin gallate (EGCG) by employing polyamide/silica gel column chromatography. The raw material was commercial tea polyphenols. The operating conditions of the chromatographic steps were investigated, first preliminary separation of EGCG by employing polyamide column chromatography, and then preparation of high purity of EGCG by employing silica gel column chromatography. The purification of EGCG is over 98% by using relative low level tea polyphenols (70% TP) for raw materials in this process.
出处
《食品与机械》
CSCD
北大核心
2009年第4期83-85,共3页
Food and Machinery
基金
四川省教育厅自然科学科研基金项目(编号:07ZC042)