摘要
目的:研究离体大鼠心脏缺血预处理对G蛋白的影响。方法:免疫印迹法测定心肌细胞膜Giα2、Giα3和Gsα含量;放射免疫法测定腺苷酸环化酶活性。结果:与对照组相比,缺血再灌注组心功能受到明显损伤,缺血预处理组心功能显著恢复。各组心脏Gsα含量无明显变化;缺血再灌注组Giα含量明显升高,腺苷酸环化酶活性降低;缺血预处理组与缺血再灌注组相比Giα2和Giα3含量分别下降41.3%和34.5%,腺苷酸环化酶活性升高。结论:缺血再灌注可引起腺苷酸环化酶信号转导系统功能障碍,缺血预处理可明显减轻缺血再灌注损伤,使细胞膜信号转导功能恢复。
ABSTRACT Objective: To study the alteration of G proteins in ischemic preconditioning. Methods: Levels of Gsα, Giα2 and Giα3 in sarcolemma were measured by Western blot analysis. Adenylyl cyclase activities were determined by radio immunoassay. Results: Cardiac function parameter decreased significantly during ischemiareperfusion, and increased significantly during ischemic preconditioning. Expressions of Gsα were unchanged. During ischemiareperfusion, contents of Giα2 and Giα3 increased, and adenylyl cyclase activities decreased. During ischemic preconditioning, contents of Giα2 and Giα3 decreased 41.3% and 34.5% respectively compared with ischemiareperfusion. Adenylyl cyclase activities increased. Conclusion: Ischemiareperfusion may contribute to the dysfunction of adenylyl cyclase signal transduction system. Ischemic preconditioning can attenuate ischemiareperfusion injury and improve the function of adenylyl cyclase signal transduction system.
出处
《北京医科大学学报》
CSCD
1998年第4期333-334,339,共3页
Journal of Peking University(Health Sciences)
基金
高等学校博士学科点专项科研基金