孤啡肽在原发性高血压时的改变及其降压机制初探

Changes of a New Endogenous Opioid-like PeptideNociceptin in Hypertensive Patients and its Hypotensive Mechanism

目的观察原发性高血压患者血浆孤啡肽水平的改变及孤啡肽对血管内皮舒张因子（ＥＤＲＦ／ＮＯ）的影响，初步探讨孤啡肽舒张血管，降低血压的可能机制。方法应用放射免疫测定法测定原发性高血压患者（３２例）血浆孤啡肽水平并以正常成年人（１９例）作对照。大鼠主动脉条组织孵育液中亚硝酸盐的含量可反映一氧化氮（ＮＯ）的产生量，采用微盘测定法测定，用张新波等建立的ＮＯ合酶测定改良法检查ＮＯＳ活性。结果ＥＨ患者血浆孤啡肽水平明显高于对照组（１５．４７±２．１４ｎｇ／Ｌｖｓ８．８３±５．６８ｎｇ／Ｌ，Ｐ＜０．０５），且随高血压程度分期的加重，孤啡肽水平增加越明显。孤啡肽能明显刺激大鼠主动脉条组织ＮＯ产生量和提高ＮＯＳ活性，随剂量的增加，此作用更加明显（Ｐ＜０．０５）。结论推测孤啡肽可能为一种存在于外周血液中与血压调节有关的新型血管活性多肽，其与心血管疾病的关系有待进一步深入研究。

Aim\ Nociceptin/Orphanin FQ is a newly discovered endogenous ligand for the opioidlike Gprotein coupled receptor(ORL 1).\ This study is to investigate the changes of nociceptin in patients with essential hypertension(EH) and its hypotensive mechanism.\ Methods\ The level of nociceptin in plasma from EH is studied by radioimmunoassay with healthy population as control.\ In additionally,the effect of nociceptin on the isolated aortic ring tissues are assessed by EDRF/NO(endothelium derived relaxing factor/nitric oxide) production and NO synthase(NOS) activity.\ Results\ The level of plasma nociceptin in EH is much higher than that in normal control(1547±214 ng/L vs 883±568 ng/L,P<005).\ Nociceptin can stimulate the aortic ring tissues′ NO production and NOS activity in a dosedependent manner.\ Conclusion\ The above results suggest that endogenous nociceptin possesses biologic activity outside CNS and may be a new vasoactive substance that may contribute to the regulation of blood pressure.