砷对人皮肤角质形成细胞抗氧化能力影响

Effect of arsenic on antioxidative ability of human keratinocytes

目的研究亚砷酸钠(NaAsO2)对人皮肤角质形成细胞(HaCaT)抗氧化能力的影响。方法用流式细胞仪检测细胞内二氯荧光素(DCF)的荧光强度;用改良硫代巴比妥酸荧光法测定细胞内丙二醛(MDA)含量;用Nitrite-kit法检测超氧化物歧化酶(SOD)活性;用紫外速率直接法测定过氧化氢酶(CAT)的活性。结果各实验组(2.5,5,10和20μmol/LNaAsO2)的DCF荧光强度均显著增高(P<0.05),分别是对照组的1.3,1.6,1.7和1.8倍;各实验组的MDA含量显著增高(P<0.05),分别是对照组的1.1,1.4,1.5和2.2倍;10和20μmol/LNaAsO2组SOD活性显著下降,分别为对照组的78%和61%;10和20μmol/LNaAsO2组SIOD和CAT活性显著下降,分别为对照组的50%和34%。结论砷可以引起人皮肤细胞的氧化损伤,降低抗氧化能力。

Objective To explore the effect of sodium arsenite（ NaAsO2 ） on antioxidative ability in human keratinocytes （HaCaT）. Methods The fluorescence intensity of dichlorofluorescein （DCF） was detected by flow cytometry. The malondialdehyde（MDA） content was detected by improved thio-barbituric acid fluorometric method. Superoxide dismutase （SOD） activity was determined by Nitrite-kit. The catalase（CAT） activity was determined by ultraviolet velocity direct method. Results The fluorescence intensity of DCF increased to 1.3,1.6,1.7, and 1.8 times of the control＇s in 2. 5,10, and 20 μmol/L NaAsO2 treated groups, respectively （ P 〈 0. 05 ）. The MDA content increased to 1.1,1.4,1.5, and 2.2 time of the control＇s in 2. 5,10,and 20 μmol/L NaAsO2 treated groups,respectively （P 〈0.05）. While the activity of SOD decreased to 78% and 61% of the control＇s in 10μmol/L and 20μmol/L NaAsO2 groups. And CAT activity decreased to 50% and 34% of the control＇s in 10p, mol）L and 20μmol/L NaAsO2 groups. Conduslon Arsenic can cause oxidative damage and decrease antioxidative ability in human kemtinocytes.