加工番茄根系分泌物自毒效应的研究

The Study on Autotoxic Effect from Root Exudates of Processing Tomato

通过提取加工番茄根系分泌物酸性、碱性和中性组分,运用化感作用原理,采用室内培养,研究其对种子萌发和幼苗生长的影响。结果表明,处理后的加工番茄种子的发芽率和发芽指数明显降低,且随着根系分泌物溶液浓度的增加,受抑制程度越显著。酸溶性根系分泌物对种子萌发的影响最大,中性根系分泌物的影响次之,碱溶性根系分泌物的影响最小,其平均发芽率分别比对照降低14.7%、11.7%和8.3%。浓度为50%的根系分泌物溶液的抑制作用最强,发芽率比对照降低了19.33%。3种组分根系分泌物对加工番茄幼苗的生长均有不同程度的抑制,降低了根系活力,比对照降低了51.9%~59.6%;而且根系分泌物使加工番茄叶片SOD酶活性降低、MDA生成量增多、POD酶活性升高,说明加工番茄根系分泌物具有自毒作用。

The paper attempts to study seed germination and seedling growth with the help of the role of a sense of principle as well as indoor training by means of extraction of acid test, alkaline and neutral processing tomato root exudates. Seed germinating rates and index decreased significantly after processed, and with the concentration of root exudates＇ solution, the degree of suppression becomes more obvious. The root of acid secretion has the largest impact, next root exudates neutral and last the basic root exudates, the germination rates were lower than control of 14.7%, 11.7% and 8.3%. Compared several concentrations, 50% solution of root exudates owns the most powerful suppression. The germination rates were lower than the control 19.33%. The growth of processed tomato seedlings has varied under the function of the three components, which mainly reduce the root system （lower than the control 51.9%-59.6%） ; and syndromes of suppression of root exudates to processed tomato leaves include lower SOD activity, increased production of MDA, and increased activity of POD, which shows that the processed tomato root exudates has the function of self-toxicity.