渭北旱塬葡萄根际解磷细菌的筛选及其对葡萄的促生效应

Screening and Promotion Effect of Phosphate-solubilizing Bacteria from Grapevine Rhizosphere in Weibei Semi-arid Plateau

对渭北旱塬50年生葡萄园解磷细菌进行了筛选,并对两个菌株的促生效应进行了研究。通过NBRIP平板,从葡萄根际土壤中分离得到29株解磷细菌。通过NBRIP液体摇瓶试验,培养7 d后得到两株解磷效果突出的菌株:JYR-12菌株培养液中有效磷含量高达673.99 mg/L,约有13.17%的磷酸钙被溶解出来;JYR-7菌株培养液中有效磷浓度为671.64 mg/L。在盆栽条件下,研究解磷细菌JYR-12和JYR-7对酿酒葡萄赤霞珠扦插苗生长及光合能力的影响。盆栽试验表明,接种解磷细菌处理的赤霞珠扦插苗株高、茎粗、地下干质量和地上干质量显著高于对照。葡萄扦插苗的光合指标及接种解磷细菌的均显著高于对照。可见,与对照相比,接种解磷细菌可以显著促进赤霞珠扦插苗生长,其中以菌株JYR-12效果最为明显。

Phosphate-solubilizing bacteria were screened and promotion effects on grapevine were studied. By using NBRIP plate method, 29 strains of phosphate-solubilizing bacteria （PSB） were isolated from the grapevine rhizosphere. After seven days incubation in a liquid medium NBRIP, the soluble P in the cultures of strains JYR-12 and JYR-7 reached 673.99 mg/L and 671.64 mg/L respectively. A- bout 13.17% of tricalcium phosphate was dissolved. In pot experiment, the growth of Cabernet sauvignon after inoculation with phosphate-solubilizing bacteria was studied. The results showed that the plant height, stem thickness, shoot dry weight and root dry weight were significantly higher in PSB treatments than in CK. After the inoculation with phosphate-solubilizing bacteria, photosynthesis indexes of grape were obviously higher than those of contrast plants. In conclusion, the inoculation of phosphate-solubilizing bacteria could significantly promote the growth of Cabernet sauvignon. Among all these inoculation methods, the best is JYR-12.