摘要
目的:建立人血浆中别嘌呤醇及其代谢产物氧别嘌醇的高效液相色谱测定方法。方法:色谱柱为Hypersil BDS C_(18)柱(250mm×4.6mm,5μm),流动相为50mmol·L^(-1)KH_2PO_4(pH 4.63)-甲醇(97:3),流速1ml·min^(-1),柱温30℃,紫外检测波长254nm。以阿昔洛韦为内标,血浆样品用高氯酸沉淀蛋白后分析。结果:别嘌呤醇在0.02～5μg·ml^(-1)范围内线性关系良好(r=0.999 2),提取回收率为76.4%～81.4%,方法回收率为98.7%～112.7%;氧别嘌醇在0.02～8μg·ml^(-1)范围内线性关系良好(r=0.999 6),提取回收率为76.3%～80.2%,方法回收率为97.2%～100.2%。结论:本法操作简便、灵敏、准确。
Objective ： To develop a method for the determination of the concentration of aUopurinol and its metabolite-oxypurinol in human Plasma by HPLC. Method： The Hypersil BDS C18 column（250mm ×4.6mm, 5μm） was used at 30℃. The mobile phase was 50 mmol · L^-1 phosphate buffer solution （ pH 4.63 ） -methanol （97：3 ） , and the flow rate was 1.0 ml · min^-1. The detecting wavelength was 254nm. Acyclovir was used as internal standard. The samples were deposited by perchloric acid and then injected. Result： The linear range of allopurinol was 0.02-5 μg · ml^ -1 （ r = 0.999 2）. The extraction recovery of allopurinol was 76.4% -81.4% and the method recovery was 98.7% -112.7%. The linear range of oxypurinal was 0.02-8μg · ml^ - 1 （ r = 0. 999 6 ）. The extraction recovery of oxypurinol was 76.3% -80.2% and the method recovery was 97.2% -100.2%. Conclusion： The method was simple, sensitive and accurate.
出处
《中国药师》
CAS
2009年第8期1033-1034,共2页
China Pharmacist
