兔自体骨髓干细胞移植联合骨髓动员治疗下肢缺血的实验研究

Experimental Study of bone marrow mobillization combined with Autologous stem cell transplantation in Rabbits on chronic lower extremity ischemia

目的:探索自体骨髓干细胞移植联合骨髓动员治疗下肢缺血性疾病的可行性。方法:于2008年3月～2008年6月进行兔骨髓干细胞移植联合骨髓动员治疗下肢缺血实验研究。建立新西兰雄兔右后肢缺血动物模型,将其分为4组:实验1组(7只)单纯应用重组粒细胞集落刺激因子进行骨髓动员,实验2组(7只)取自体骨髓干细胞制备成干细胞悬液,注射于右后肢缺血部位,实验3组(7只)为自体骨髓干细胞移植后进行骨髓动员组,4组(7只)为生理盐水注射组,于动物模型制备后第5周行血管造影观察血管新生程度,CD34免疫组化检测毛细血管密度。结果:动脉造影显示进行骨髓干细胞移植联合骨髓动员的新西兰雄兔缺血右后肢缺血部位动脉较其他组明显增多,CD34免疫组化标记毛细血管密度增加。结论:自体骨髓干细胞移植联合骨髓动员是一种方法简便、安全有效的治疗下肢缺血性疾病的方法。

Objective： Explore the feasibility of chronic lower extremity ischemia treated by bone marrow mobillization combined with autologous stem cell transplantation. Methods ： performed Experimental Study of bone marrow mobillization combined with autologous stem cell transplantation in rabbits on chronic lower extremity ischemia between 2008-03 - 2008-06. By model building of New zealand male rabbit of right hind ischemia, totally four teams：the first group（ seven rabbits ） with bone marrow mobillization,the second group（ Seven rabbits ） with autologous stem cell transplantation, the third group （ seven Rabbits ） with bone marrow mobillization combined with autologous stem cell transplantation, the fourth group is control group, Observed the Level of vascular neogenesis after the fifth week of model building, testing the vascular density by CD34 immunohistochemistry. Results：We can see he vascular density increasing from DSA and CD34 immunohistochemistry, that the conbined group compare other group. Conclusion： Bone marrow mobillization combined with autologous stem cell transplantation is the convenient and safe treatment on chronic lower extremity ischemia.