小肠结肠炎耶尔森氏菌致Graves’病作用的实验研究

Studies on the relationship between Yersinia infection and the pathogenesis of Graves' disease

目的 研究小肠结肠炎耶尔森菌（ Ｙ． Ｅ 菌） 感染和 Ｇｒａｖｅｓ’病发病的相关性。方法 用 Ｏ：３ Ｙ． Ｅ 菌免疫新西兰兔，研究 Ｙ． Ｅ 菌抗体（ Ｙ Ｅ Ａｂ） 和 Ｔ Ｒ Ａｂ 的相关性及其对 Ｆ Ｒ Ｔ Ｌ５ 细胞释放ｃ Ａ Ｍ Ｐ 的影响。结果 Ｏ：３ Ｙ． Ｅ 菌免疫１２ 周后，其中４ 只兔（４／１０） 产生了 Ｙ． Ｅ 菌抗体（ Ｙ Ｅ Ａｂ） ，滴度为１ ：５０００ ～１ ：２５０００ ，同时免疫兔甲状腺功能（ Ｔ３ 、 Ｔ４） 也明显升高。交叉吸附试验表明 Ｙ Ｅ Ａｂ 和 Ｔ Ｒ Ａｂ 能竞争性地与 Ｔ Ｓ Ｈ 受体蛋白结合，另外， Ｙ Ｅ Ａｂ 阳性血清中提取的 Ｉｇ Ｇ 能刺激 Ｆ Ｒ Ｔ Ｌ５ 细胞释放ｃ Ａ Ｍ Ｐ。结论 在免疫学和细胞生物学水平证实了 Ｙ． Ｅ 菌感染与 Ｇｒａｖｅｓ’病发病有关。

Objective To study the relationship between Yersinia infection and the pathogenesis of Graves' disease. Methods Rabbits were immunized with O:3 Yersinia enterocolitica (Y.E) and then the relationship between YEAb and TRAb and YEAb's effect on cAMP release from FRTL 5 cell were studied. Results After 12 weeks, 4 of 10 rabbits immunized with O:3 Y.E developed high levels of antibodies to O:3 Y.E, the titers of YEAb being from 1:5000 to 1:25000, and their serum total T 3 and total T 4 levels increased also. The cross absorption test indicated that YEAb and TRAb were able to bind with TSH receptor competitively; besides, the IgGs prepared from YEAb positive sera had the ability to stimulate the release of cAMP from FRTL 5 cell. Conclusion All the results obtained demonstrated immunologically and cytologically the relationship between the Y.E infection and the pathogenesis of Graves' disease.