胎牛血清促进色素上皮衍生因子在皮肤角质形成细胞和成纤维细胞中的表达

Fetal bovine serum enhances expression of PEDF in epidermal keratinocytes and dermal fibroblasts

目的:探讨表皮角质形成细胞和真皮成纤维细胞中色素上皮衍生因子(PEDF)表达的影响因素。方法:体外培养角质形成细胞和成纤维细胞,并以10%FBS(胎牛血清)刺激,通过免疫荧光和Western blot检测PEDF的表达。结果:PEDF大多位于细胞浆中,但在细胞核中也有少量表达。细胞浆中PEDF并非均质型分布,而是呈细颗粒状集聚。10%FBS促进角质形成细胞和成纤维细胞中PEDF的集聚和表达,而且这一分布形式不受组胺和佛波肉豆蔻醋酸(PMA)的影响。结论:10%FBS促进角质形成细胞和成纤维细胞中PEDF的集聚和表达。

Objective： To investigate the effect of fetal bovine serum （FBS） on expression of pigment epithelium-derived factor （PEDF） in normal epidermal keratinocytes and dermal fibroblasts. Methods： Keratinocytes-and fibroblasts were incubated with 10% FBS. PEDF protein level in the cells was determined by immunofluorescence and Western blot. Results： PEDF was localized mostly in the cytoplasm,while some in the nuclei. The distribution of PEDF in cytoplasm was in a-granular pattern. 10% FBS increased the expression of PEDF both in keratinocytes and fibroblasts,but histamine and Phorbol 12-myristate 13-acetate （PMA） did not interfere the distribution of PEDF in cells. Conclusion： 10% FBS can upregulate expression of PEDF in epidermal keratinocytes and dermal fibroblasts.