“醒脑开窍”针刺法对脑缺血大鼠脑皮质Peroxiredoxin Ⅵ表达的影响

The Effect of "Xingnao Kaiqiao" Acupuncture on the Expression of Peroxiredoxin Ⅵ in the Ischemia Cortex of Rats

目的探讨脑缺血大鼠皮质抗氧化蛋白Ⅵ(peroxiredoxinⅥ)的表达以及"醒脑开窍"针刺法的干预作用。方法采用大鼠大脑中动脉阻塞(MCAO)模型,通过western blotting方法,检测醒脑开窍针刺组、对照组(传统针刺组)、模型组,假手术组PeroxiredoxinⅥ的表达。结果6h时,醒脑开窍组与模型组比较表达明显升高(P<0.05);24h时,醒脑开窍组和对照组与模型组比较都有显著的升高(P<0.05)。结论结果提示"醒脑开窍"针刺法能够提高大鼠脑缺血后PeroxiredoxinⅥ的表达,在缺血早期效果优于传统针刺法,对提高大鼠脑缺血后的抗氧化能力有积极意义。

Objective To investigate and observe the expression of Peroxiredoxin Ⅵ in ischemia cortex of rats and the intervention effect of Xingnao Kaiqiao Acupuncture（XKA） therapy. Methods Through western blotting,we detected the expression of Peroxiredoxin Ⅵ in xingnao kaiqiao group , comparison group, model group and sham operation group in middle cerebral artery occlusion （MCAO）model of rats. Results At 6h, the expression of Peroxiredoxin VI in xingnao kaiqiao group , comparison group and sham operation group was increased than that in moddle group,of which Xingnao Kaiqiao group increased obviously （ P 〈 0.05 ）. At 24h, as compared with modle group, the expression of Peroxiredoxin Ⅵ in xingnao kaiqiao group and comparison group increased significantly （P 〈 0.05）. Conclusion Xingnao Kaiqiao Acupuncture therapy could raise the expression of Peroxiredoxin Ⅵ in focal cerebral ischemia of rats, and the effect is better than that of traditional acupuncture therapy in the earlier period. It is benefit to elevating the ability of antioxygen of focal cerebral ischemia of rats.