Genistein-磁性纳米微粒对人肝癌细胞中FAK表达的影响

Effects of Genistein-magnetic-nanoparticles on the Expression of FAK in Human Hepatocellular Carcinoma Cell line HepG_2

目的 观察不同浓度genistein-磁性纳米微粒对肝癌细胞株HepG2生长及FAK表达的影响。方法 四甲基偶氮唑盐（MTT）法测定genistein-磁性纳米微粒作用的肝癌细胞株HepG2活性，RT—PCR及免疫组化法分别检测FAK mRNA及FAK蛋白的表达。结果 HepG，细胞经genistein-磁性纳米微粒（浓度范围10～80mg／L）作用后细胞生长明显受到抑制，具有剂量依赖效应关系和时间依赖效应关系（P〈0．05）。RT—PCR检测不同浓度genistein-磁性纳米微粒作用48h后FAK mRNA表达的相对水平分别为1．242±0．031，1．213±0．443，0．834±0．044，0．652±0．039，0．446±0．041，对照组为1．443±0．053（F=21．97，P〈0．05），FAK蛋白表达明显减弱，也有明显的剂量效应关系。结论 genistein-磁性纳米微粒能够降低肝癌细胞FAK—mRNA及蛋白的表达，这作用可能是其抑制肝癌细胞的生长的重要机制之一。

Objective To study the effects of Genistein - magnetic - nanoparticles at different concentrations on the growth and expression of focal adhesion kinase （FAK） in human hepatocellular carcinoma cell line HepG2. Methods Activities of HepG2 ceils treated by Genistein - magnetic - nanoparticles were examined by MTT assay. The expression of FAK mRNA and protein was respectively detected by immunohistochemistry staining and reversed transcriptase polymerase chain reaction （ RT - PCR） . Results Genistein - magnetic - nanoparticles at a concentration of 10 -80mg/L inhibited the proliferation of HepG2 cells, with obvious concentration - dapendent and time - dependent effect relationships （P 〈 0.05）. After treatment with various concentrations of Genistein - magnetic - nanoparticles for 48h, the relative level of FAKmRNA of Genistein - magnetic - nanoparticles and control groups was 1. 242 ± 0.031,1. 213 ± 0. 443,0. 834 ± 0. 044,0. 652 ± 0. 039,0. 446 ± 0. 041, and 1. 443 ± 0. 053 （ F = 21.97 ,P 〈 0.05 ）, respectively. The expression of FAK protein in the cells was decreased, which showed an obvious a concentration - effect relationship. Conclusion Genistein - magnetic - nanoparticles can reduce the mRNA and protein expressions of FAK and inhibit the proliferation of HepG2 cells.