内毒素休克兔血浆NO和ET-1水平的变化及相互关系

Dynamic alterations and interaction of plasma NO and ET-1 levels in rabbit endotoxic shock

目的与方法：用兔内毒素休克模型，观察血浆ＮＯ和内皮素－１（ＥＴ－１）浓度的动态变化，探讨两者的相互影响。结果：ＬＰＳ组血浆ＮＯ浓度于ｉｖＬＰＳ后１２０ｍｉｎ升高，１８０ｍｉｎ达峰；ＥＴ－１升高较早，６０ｍｉｎ达峰，随后衰减。Ｌ－ＮＭＡ抑制ＮＯ和ＥＴ－１生成，而ｐｈｏｓｐｈｏｒａｍｉｄｏｎ只抑制ＥＴ－１生成，两者均纠正ＬＰＳ引起的ＳＶ下降。Ｄｅｘ组血浆ＮＯ与ＥＴ－１含量波动较小。结论：ＥＴ和ＮＯ是参与内毒素休克不同时相的体液因子，且一定浓度的ＮＯ是ＥＴ－１生成的条件之一。

AIM and METHOD:The dynamic changes of concentration of plasma NO and endothelin-1(ET-1) were investigated in endotoxic shock of rabbits.RESULTS:In LPS group,the plasma concentrations of both NO and ET-1 increased significantly,but their temporal dynamics were different.The increase of plasma NO concentration started at 120 min after endotoxin injection and reached its peak at 180 min.While plasma ET-1 concentration increased much earlier:at 60 min post-endotoxin and its plasma concentration had reached its peak level,then waned. L-NMA inhibited both the increase of NO and ET-1 concentrations in plasma,but phosphoramidon only alleviated the rise of the plasma ET-1 content.Dex group's NO and ET-1 level did not change.CONCLUSION:NO and ET-1 might be an important liquid factor in different phases of endotoxic shock and NO was essential for ET-1 increase during endotoxic shock.