摘要
水培65d的烟株进行打顶后,测定打顶和不打顶烟株上部叶中烟碱含量。分别提取根中总RNA,以腐胺N-甲基转移酶基因(PMT)特异引物、肌动蛋白基因(actin)为内参作半定量RT-PCR分析;同时将提取的总RNA转膜,用地高辛标记PMTRT-PCR产物作探针,进行Northern杂交分析的结果表明,打顶后的上部烟叶中烟碱含量和PMT基因表达量显著增加。
The tobacco （Nicotiana tabacum） plants were topped after water cultured for 65 d and the top leaf nicotine contents of the topped and untopped were examined. Total RNA were extracted respectively from the roots of topped and untopped tobacco plants. Expression analysis of putrescine N-methyltransferase gene （PMT） was carried out by semi-quantitative RT-PCR with the tobacco actin as control and by Northern blotting with the digoxin-labelled PMT RT-PCR product as probe. Results showed that PMT expression and the nicotine content in the top leaf increased after topping.
出处
《植物生理学通讯》
CSCD
北大核心
2009年第7期684-686,共3页
Plant Physiology Communications
基金
国家烟草专卖局重点项目(110200001011A)
