大鼠髓核自身免疫反应的研究

A study on autoimmune properties of rat's pucleus pulposus

目的探讨动物模型中将自身髓核埋置在皮下腔隙,是否能引起T、B淋巴细胞的激活。方法从12只大鼠的椎间盘中取得自身髓核组织,放置在有孔的钛笼内,植入皮下。在每只大鼠的皮下还植入一个空的肽笼作为对照。7d后处死大鼠并取出钛笼。使用流式细胞术检测钛笼内外周组织中的辅助T细胞(CD4+),细胞毒性T细胞(CD8+),CD45RC+高表达的T细胞以及IgG+的B细胞。结果与空的钛笼作比较,装有自身髓核组织钛笼的周围组织中,活化的T细胞(CD4+/CD8+)、B淋巴细胞(IgG+)以及CD45RC+高表达的T细胞的比例明显增高。结论大鼠自身髓核组织暴露于自身免疫系统中,可激活T、B细胞,引起自身免疫反应。

Objective To study if subcutaneously placed autologous nucleus pulposus can activate T and B cells in an animal model. Methods Autologous nucleus pulposus was harvested from the intervertebral disc of 12 rats and placed subcutaneously in perforated titanium chambers. An additional empty chamber was placed subcutaneously in each rat as the control. The rats were killed and the chambers were harvested 7 days late. Flow cytometry was used for detecting T-helper cells （CIN＋ ） ,cytotoxic T cells （CD8＋ ） ,T cells （CD45RC＋ ）and B cells （IgG＋ ） in the tissues around the chambers. Results Compared with the tissues around the empty chambers, the proportion of activated T cells （CD＋ and CD8＋ ）, higher amount of T cells （CD45RC＋ ） and the proportion of activated B cells（IgG＋ ） was significantly higher in the nucleus pulposus filled chambers. Conclusion The findings indicate that autologous nucleus pulposus can activate T and B cells, and rigger an autoimmune response if exposed to the immune system.