灵芝多糖对失血性休克复苏时肺损伤的保护作用

Protective effect of GLP on lung injury induced by hemorrhagic shock-reperfusion in rabbits

目的探讨灵芝多糖(GLP)对失血性休克复苏时肺损伤的保护作用。方法复制家兔失血性休克再灌注(SH-R)复苏模型,随机分成假手术组(S组)、生理盐水再灌注复苏组(NS组)和质量分数为1%的GLP再灌注复苏组(LS组)。于再灌注复苏90min时快速处死动物,观察肺组织一氧化氮(NO)含量和一氧化氮合酶(NOS)活性与肺组织损伤性变化的关系。结果NS和LS组肺组织NOS活性、NO含量均明显低于S组,但LS组明显高于NS组(P<0.05);NS和LS组肺组织湿/干比(W/D)、损伤肺泡百分率(IAR)和凋亡细胞百分数、支气管肺泡灌洗液(BALF)中白细胞计数和肺通透指数(PPI)明显高于S组,而LS组明显低于NS组(P<0.05);病理显示:NS组肺损伤明显,LS组肺损伤不明显。结论GLP可通过激活NOS、增加NO含量、减少多形核白细胞(PMN)在肺部的集聚而对SH-R肺损伤起保护作用。

Objective To study the protective effect of ganoderma lucidum polysaccharide （GLP） on lung injury induced by hemorrhagic shock-reperfusion in rabbits. Methods The rabbits were divided into group S（sham operation）, group NS（reperfusion with normal saline for 90 min after hemorrhagic shock）,and group LS（reperfusion with 1% GLP instead of NS as in group NS）. The rabbits were killed quickly at the end of reperfusion for measuring NO content and activity of NO synthase（NOS） and pathological examination. Results The activity of NOS and the content of NO in the lungs were lower in groups of NS and LS than those in group S, which were higher in group LS than those in group NS （P〈0.05）. Leucocyte count in bronchoalveolar lavage fluid was more in group NS than that in group S and group LS （P〈0. 05）. The pathological results showed serious pulmonary structural injury occurred in group NS, but no significant injury was found in group LS. Conclusion GLP has a protective effect on lung reperfusion injury induced by HS-R through enhancing NOS activity and increasing NO production.