The mixture of procyanidins extracted from the lotus seed pod and bilobalide ameliorates scopolamine-induced memory impairment in mice

莲房原花青素和银杏内酯联用对东莨菪碱所致小鼠记忆获得性障碍的改善作用(英文)

Objective To study the co-effect of procyanidins extracted from the lotus seed pod （LSPC） and bilobalide （BIL） on ameliorating scopolamine-induced learning and memory impairment in young mice. Methods Fifty male Kunming mice with similar learning and memory capabilities were selected by Morris water maze test and were randomized into 5 groups （n=10 in each group）： control group, scopolamine group, L-（LSPC＋BIL） group （50 mg/kg LSPC＋10 mg/kg BIL）, M-（LSPC＋BIL） group （100 mg/kg LSPC＋20 mg/kg BIL）, H-（LSPC＋BIL） group （150 mg/kg LSPC＋30 mg/kg BIL）. Scopolamine model with impaired learning and memory was established by scopolamine treatment （1 mg/kg）, and after 10 min mice were tested. In L-, M-, and H- （LSPC＋BIL） groups, mice were treated with LSPC and BIL ig. for 30 days, while mice in the other 2 groups were treated with normal saline ig. instead. After the 30-day＇s treatment, the co-effect of LSPC and BIL on learning and memory was tested by Morris water maze and the step-down avoidance tests. Results The memory impairment caused by scopolamine in young mice could be ameliorated by co-treatment of LSPC and BIL, as indicated by significantly shorter escape latency and swimming distance in the Morris water maze test, when compared with those in the scopolamine group. In the step-down avoidance test, mice in all the 3 dose groups showed significantly smaller number of errors and longer latency than mice in the scopolamine group did. Conclusion Co-treatment of LSPE and BIL can ameliorate scopolamine-induced learning and memory impairment in young mice.

目的观察莲房原花青素和银杏内酯联用对东莨菪碱所致小鼠学习记忆获得性障碍的改善作用。方法采用Morris水迷宫筛选出50只空间学习记忆能力相近的昆明种雄性小鼠,随机分为5组,即正常对照组、模型组、低剂量治疗组、中剂量治疗组、高剂量治疗组,每组10只。行为学测试前10min腹腔注射东莨菪碱,建立小鼠记忆获得障碍模型。3个治疗组分别灌胃50mg/kg莲房原花青素+10mg/kg银杏内酯、100mg/kg莲房原花青素+20mg/kg银杏内酯、150mg/kg莲房原花青素+30mg/kg银杏内酯;正常组和模型组以生理盐水代替。喂养30天后以Morris水迷宫法、跳台法行为学实验,观察莲房原花青素和银杏内酯的联同作用。结果在东莨菪碱所致小鼠记忆障碍模型中,莲房原花青素和银杏内酯联用可缩短在Morris水迷宫中的潜伏期,减少游泳距离。在跳台实验中莲房原花青素和银杏内酯联用可以延长平台停留期,显著减少错误次数。结论莲房原花青素和银杏内酯联用能够改善东莨菪碱所致小鼠学习记忆障碍。