RP-HPLC法测定不同产地荜澄茄中芦丁的含量

Determination of the content of rutin in Litsea cubeba by RP-HPLC

目的建立测定不同产地荜澄茄中芦丁含量的RP-HPLC法。方法采用Kromasil C18色谱柱（250 mm×4.6 mm,5μm）,以甲醇-体积分数为0.05%的磷酸（体积比为38∶62）为流动相,流速为1 mL.min-1,检测波长为254 nm。结果芦丁的质量浓度在1.664-62.400 mg.L-1（r=0.999 4）内与色谱峰面积呈良好线性关系,平均回收率为101.2%（RSD=2.3%）。结论该方法可作为该药材质量控制的一项指标。

Objective To describe a method for determining rutin in Litsea cubeba by RP-HPLC. Methods The determination was carded out over Kromasil C18 column（250 mm ×4. 6 mm,5μm）. The mobile phase consisted of methanol- 0. 05% （w） phosphoric acid aqueous solution （ V： V = 38 ： 62 ）. The flow rate was 1mL. min - 1, and detection wavelength was set at 254 nm. Results Good linearity was observed in the range of I. 664- 62. 400 mg· L^-1 （ r = 0. 999 4 ） for rutin. The average recovery of rutin was 100. 5% （ RSD - 1.0% ）. Conclusions The method is simple, accurate and reliable; it can be used for the quality control of Litsea cubeba.