体外构建携带PDGF-BB和IGF-1缓释微粒的脱细胞人工真皮

An Experimental Study on Reconstruction of Acellular Dermal Matrix Carried PDGF-BB and IGF-1

目的探讨体外构建携带并持续释放血小板源生长因子(PDGF-BB)和胰岛素样生长因子(IGF-1)的脱细胞人工真皮的可行性。方法体外合成含PDGF-BB和IGF-1生长因子的可降解微球,并与脱细胞人工真皮联结,Elisa方法检测人工真皮中生长因子释放情况;通过携带生长因子的人工真皮与真皮成纤维细胞的共培养,采用细胞计数及WST-8比色法,观察成纤维细胞的增殖速率与细胞活力。结果携带生长因子的人工真皮与真皮成纤维细胞共培养组与对照组细胞倍增时间分别为(2.01±0.38)d和(7.27±1.16)d,共培养组细胞倍增时间明显缩短(P<0.05,n=5);体外培养7d、14d、21d时,共培养组及对照组细胞活力无统计学差异(P>0.05,n=5)。结论本实验成功制备了能持续释放PDGF-BB和IGF-1生长因子并加速体外培养的成纤维细胞增殖的人工真皮,为加速创面修复的研究提供实验依据。

Objective To explore the reconstruction of acellular dermal matrix carried PDGF-BB and IGF-1 in vitro. Also investigate cultured human fibroblast proliferation and collagen production affected by constructed aeelluar dermal matrix. Methods The degraded micro beads with PDGF-BB and IGF-1 were synthe-sized and connected to aeellular dermal matrix. Acelluar dermal matrix was euhured with fibroblast. Fibroblast proliferation rate and cell variability were observed by eell count and WST-8 colorimeter; The release of PDGF-BB and IGF-1 growth factors were measured by ELISA. Results The population doubling time of acellular dermal matrix fibroblast co-culture group and control group was 2.01±0.38 days and 7.27±1.16 days in experimental group and control group respectively. There was significant difference （P〈0.05）. But there was no statistically significant difference in cell viability after culture 7, 14, 21 days. Conclusion Acelluar dermal matrix carried PDGF-BB and IGF-1 micro beads can promote the proliferation of fibroblast with continually release of PDGF-BB and IGF-1 growth factors so it is an effective mothod for wound repair.